Development cooperation no longer lies only in the hands of public authorities. While non-governmental organisations might be the most traditional counterpart of governments in international aid and development cooperation in many countries, they are experiencing the rise of other players. This changing context brings with it opportunities to create new and innovative alliances to pool knowledge and resources for mutual benefit. Following on from the publication “Shaping a new generation of decentralised cooperation for enhanced effectiveness and accountability” (CPMR & PLATFORMA, 2017) this second study looks at how regional and local governments can be instrumental in bringing together different stakeholders in efficient, coherent and complementary partnerships for decentralised cooperation. The study draws on concrete examples and case studies, including best practices from several CPMR member Regions, to provide recommendations and proposals for reinforcing these multi-stakeholder partnerships for decentralised cooperation in the context of the 2030 Agenda and the EU’s aims to enhance the territorial dimension.